Journal: CNS Neuroscience & Therapeutics

Article Title: Identification of a Novel Radiosensitivity‐Related Signature and Validation of GPX8 in Regulating the Radiosensitivity of Glioma

doi: 10.1002/cns.70900

Figure Lengend Snippet: Suppression of GPX8 boosts the radiosensitivity of glioma cells in vivo. A, B. Validation of GPX8 knockdown efficiency in U87 stable cell lines generated via lentivirus‐mediated shRNA. (A) RT‐qPCR analysis. (B) Western blotting analysis. C. Experimental scheme for the U87 subcutaneous xenograft tumor model in BALB/c‐nude mice and radiotherapy treatment. D. Comparison of tumor volumes across the four experimental groups under different treatments. E. Growth curves of U87 subcutaneous xenograft tumors in the four mouse groups following radiotherapy. F. Comparison of excised tumor weights from the four treatment groups. G. Immunohistochemical (IHC) staining of mouse tumor tissues for GPX8, Ki‐67, γ‐H2AX, F4/80, and NKp46 expression (20×: Scale bar = 100 μm). H‐L. Quantitative analysis of IHC staining intensity for the respective markers. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns means no statistical significance.

Article Snippet: According to the manufacturer's instructions, we used jetPRIME siRNA transfection reagent (Polyplus, France) to transfect U251 and LN229 cells and collected total RNA and protein for detection 48‐72 h after transfection.

Techniques: In Vivo, Biomarker Discovery, Knockdown, Stable Transfection, Generated, shRNA, Quantitative RT-PCR, Western Blot, Comparison, Immunohistochemical staining, Immunohistochemistry, Expressing